ImageFocus Alpha vs Open Galileo

ImageFocus Alpha is microscope image capture and analysis software from Euromex. It supports measurements and annotations on live views and captured images, with tools for segmentation, counting, image stitching, and extended depth of focus. Fluorescence functions include combining captured fluorescence images. Images can be saved as.jpg,.tif,.bmp, or.dicom files, and videos as.avi files. Euromex provides installations for Windows, Mac OS, and Linux, as well as Android and iOS apps for Wi-Fi enabled devices. The full package includes DirectShow, Twain, and µManager drivers. The Alpha download is designed for compatible Euromex equipment, including USB-3 Pro and scientific cameras, cooled and Wi-Fi cameras, HD-Pro and other VC cameras, a digital bScope, and DC.2000L. The listed ImageFocus Alpha plan is free, and Euromex says software updates are available free from its website. The iOS app is available only through the App Store. Euromex describes the software as supplied with camera solutions for universities, laboratories, and industry, and directs customers to its downloads page for assistance.

Open Galileo is free, open-source Windows software for focus stacking and signal integration in scientific photography, microscopy, macro photography, astrophotography, and technical documentation. It automatically aligns image sets with correlation algorithms, compensating for focus breathing and small shot differences. Focus fusion options include PMax multiband, weighted average, and depth-map methods. Astronomy mode combines signals using mean, median, sum, or sigma-clipping integration. Processing uses Laplacian, Difference of Gaussians, and variance focus measures, plus multiscale fusion with Laplacian pyramids. Optional tools provide contrast normalization, CLAHE, Unsharp Mask sharpening, and noise reduction including BM3D and approximate Noise2Void. Folders can be processed and saved automatically. JPG, PNG, TIFF, and RAW files are supported. The interface includes live previews, progress bars, parameter sliders, and a large-screen layout. It is released under CC BY-NC 4.0; commercial use needs the author's prior permission.