ImageFocus Alpha vs OpenFocus

ImageFocus Alpha is microscope image capture and analysis software from Euromex. It supports measurements and annotations on live views and captured images, with tools for segmentation, counting, image stitching, and extended depth of focus. Fluorescence functions include combining captured fluorescence images. Images can be saved as.jpg,.tif,.bmp, or.dicom files, and videos as.avi files. Euromex provides installations for Windows, Mac OS, and Linux, as well as Android and iOS apps for Wi-Fi enabled devices. The full package includes DirectShow, Twain, and µManager drivers. The Alpha download is designed for compatible Euromex equipment, including USB-3 Pro and scientific cameras, cooled and Wi-Fi cameras, HD-Pro and other VC cameras, a digital bScope, and DC.2000L. The listed ImageFocus Alpha plan is free, and Euromex says software updates are available free from its website. The iOS app is available only through the App Store. Euromex describes the software as supplied with camera solutions for universities, laboratories, and industry, and directs customers to its downloads page for assistance.

OpenFocus aligns and fuses multi-focus image sequences to create images with focus throughout. It offers ECC and Homography alignment and several fusion methods: Guided Filter, DCT, DTCWT, GFG-FGF and the StackMFF V4 AI model. A wipe view lets users compare source frames with results, and the software can generate depth maps showing focus position per pixel. It supports folder-based batch processing and a headless command-line interface for scripting and CI. Listed inputs include JPG, PNG, BMP, TIFF, MP4 and numbered image folders; the manual also describes decoding additional video formats. Outputs include those image formats, image-sequence folders and GIF animations. Research functions include metadata-calibrated scale bars and multi-page TIFF stacks that preserve 16-bit depth. The interface supports English, Chinese, Japanese and Spanish, with automatic system-language detection. OpenFocus is free under the MIT License, though included algorithms may have separate license terms. Its Windows and macOS app builds are unsigned and may trigger SmartScreen or Gatekeeper warnings on first launch.