ImageFocus Alpha vs PICOLAY

ImageFocus Alpha is microscope image capture and analysis software from Euromex. It supports measurements and annotations on live views and captured images, with tools for segmentation, counting, image stitching, and extended depth of focus. Fluorescence functions include combining captured fluorescence images. Images can be saved as.jpg,.tif,.bmp, or.dicom files, and videos as.avi files. Euromex provides installations for Windows, Mac OS, and Linux, as well as Android and iOS apps for Wi-Fi enabled devices. The full package includes DirectShow, Twain, and µManager drivers. The Alpha download is designed for compatible Euromex equipment, including USB-3 Pro and scientific cameras, cooled and Wi-Fi cameras, HD-Pro and other VC cameras, a digital bScope, and DC.2000L. The listed ImageFocus Alpha plan is free, and Euromex says software updates are available free from its website. The iOS app is available only through the App Store. Euromex describes the software as supplied with camera solutions for universities, laboratories, and industry, and directs customers to its downloads page for assistance.

PICOLAY is a free Windows program for improving images made from picture series. Its focus-stacking process combines sharp areas from photographs taken at different focus levels in a z-stack. From stacks, it can generate depth maps, 3D projections, stereo images, hologram views, and 3D animations. Editing tools cover resizing, color and sharpness adjustments, filtering, rotation, flipping, painting, cloning, text, and scale bars. It can align displaced stack images by their x and y positions and harmonize their brightness. PICOLAY runs without installation and can be copied to a folder or USB stick. Downloads include 64-bit versions and a 32-bit ZIP version. The download page labels the current release Version 2026-09-29 and lists AI-supported builds. The maker says some antivirus programs may flag the download because distribution is limited and its reputation score is weak. PICOLAY is offered as-is without warranty and may be used freely for non-commercial purposes. Manuals are available in five languages, with tutorials and FAQs linked.