ImageFocus Alpha vs Shine Stacker

ImageFocus Alpha is microscope image capture and analysis software from Euromex. It supports measurements and annotations on live views and captured images, with tools for segmentation, counting, image stitching, and extended depth of focus. Fluorescence functions include combining captured fluorescence images. Images can be saved as.jpg,.tif,.bmp, or.dicom files, and videos as.avi files. Euromex provides installations for Windows, Mac OS, and Linux, as well as Android and iOS apps for Wi-Fi enabled devices. The full package includes DirectShow, Twain, and µManager drivers. The Alpha download is designed for compatible Euromex equipment, including USB-3 Pro and scientific cameras, cooled and Wi-Fi cameras, HD-Pro and other VC cameras, a digital bScope, and DC.2000L. The listed ImageFocus Alpha plan is free, and Euromex says software updates are available free from its website. The iOS app is available only through the App Store. Euromex describes the software as supplied with camera solutions for universities, laboratories, and industry, and directs customers to its downloads page for assistance.

Shine Stacker combines images captured at different focus distances to produce images with extended depth of field. It is intended for microscopy, macro photography, and computational imaging. The software offers Laplacian pyramid and depth map stacking methods, with automatic alignment, color or luminosity balancing, noisy pixel masking, vignetting correction, and final filters. For retouching, users can select details from individual frames to address dust, ghosts, and artifacts. Batch workflows can divide many frames into smaller groups before merging them into one focused image. Python batch processing and Jupyter notebook scripting are supported. Shine Stacker supports RAW files and lists TIFF, JPEG, and multilayer TIFF as output formats. It is open source under the LGPL-3.0 license and free to download. The listed platforms are Windows, macOS, Linux, and API. The maker offers a GitHub download and Python installation through PyPI with pip.