Alternatives to Pixel Studio

LibreSprite suits people making pixel-art sprites and 2D animations who want layer, frame, palette, and export tools.

PixelWall

PixelWall suits pixel-art makers who want drawing, animation, tilemap, and scripting tools in one local workspace.

Hexcalibur suits pixel artists and voxel-game developers, including creators of Minecraft textures or projects using its listed engine exports.

Pixelc suits people making pixel art, tiles, or sprites on touch devices, desktops, or in a browser.

Manabit may suit artists and indie game developers making pixel art, tile maps or animations.

Pixelorama

Pixelorama suits people making pixel art, animations, or game graphics, including users who need tilemaps or desktop command-line exports.

Pixilart suits people creating pixel art, sprites, icons, or animated GIFs, as well as artists who want to share work and interact publicly.

Pro Motion NG

It suits artists making pixel graphics, animations, sprites, tiles or game maps, particularly for handheld systems and web games.

Pix2D

Pix2D is intended for game developers and digital artists making pixel art, sprites, or animation.